Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Cytiva HBS-P+ Buffer 10, 4x50mL
Concentrated stock solution containing 0.1 M HEPES, 1.5 M NaCl and 0.5% v/v Surfactant P20. Will yield pH 7.4 when diluted 10X. Supports the recommendations for small molecule assays in Biacore systems. For all systems.
Cytiva HBS-EP+ Buffer 10, 1000mL
General purpose running buffer. Concentrated stock solution yielding pH 7.4 when diluted 10x. For use in Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100
R&D Systems Maurice CE-SDS Running Buffer - Bottom
Running buffer for CE-SDS application. 15 mL. For use with CE-SDS cartridge (PS-MC02-S) on Maurice (090-000) and Maurice S. (090-001) systems only.
Teknova 1M HEPES, pH 7.4. 1000mL, Sterile.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M HEPES, pH 8.0. 1000mL, Sterile
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M HEPES, pH 7.5 . 1000mL, Sterile, Dnase/Rnase Tested. PCR Grade
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 10X PBS Solution. pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Cole-Parmer Oakton Traceable pH Standard Buffer with Calibration, Clear, pH 10; 500 mL
Oakton Traceable pH Standard Buffer with Calibration, Clear, pH 10; 500 mLNIST-traceable reference materials include a calibration report from an ISO 17034 and ISO 17025 accredited laboratoryChoose from clear or color-coded standards from common calibration points500 mL (16 fl.oz.) capacityCalibrate at pH 10
STEMCELL Technologies EasySep™ Buffer, Size: 1 L
EasySep™ Buffer is recommended for EasySep™ cell separation protocols.
Boston Bioproducts Inc Tris-Glycine-SDS Running Buffer (10X) - 1L
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
Bioworld Tris-HCl Buffer 10mM, pH 8.5, 500ml
Common buffer for biological sciences. Technical Information: Biotechnology grade, pH 8.5 +-0.1. Warnings: Causes irritation to eyes, skin, and mucus membranes. Do not inhale or ingest. Wash thoroughly after handling. Appearance: Liquid, PH Value: 8.5, Molecular Weight : 157.6, Molecular Formula : C4H11NO3.HCl, pH : 8.5, Solubility : Soluble in water. Appearance Form : Liquid. Appearance Color : Clear. 500ml
Bioworld Tris-HCl Buffer 10mM, pH 8.0, Sterile | 1185-53-1 | MFCD00012590 | 500mL
Tris-HCl Buffer 10mM, pH 8.0, Sterile | MW: 157.596 | 1185-53-1 | MFCD00012590 | 500mL