Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M HEPES, pH 8.0. 1000mL, Sterile
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M Tris-HCl, pH 8.5. 500mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Cole-Parmer Oakton Traceable pH Standard Buffer with Calibration, Clear, pH 10; 500 mL
Oakton Traceable pH Standard Buffer with Calibration, Clear, pH 10; 500 mLNIST-traceable reference materials include a calibration report from an ISO 17034 and ISO 17025 accredited laboratoryChoose from clear or color-coded standards from common calibration points500 mL (16 fl.oz.) capacityCalibrate at pH 10
STEMCELL Technologies Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum, Size: 500 mL
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum is recommended for use as a buffer to dilute or wash nucleated cells.
Teknova 1X PBS Solution, pH 7.4. 1000mL, Sterile
135.0mM Sodium Chloride, 2.7mM Potassium Chloride, 4.3mM Disodium Phosphate, 1.4mM Monopotassium Phosphate
Teknova 10X PBS Solution. pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Bioworld Tris-HCl Buffer 10mM, pH 8.0, Sterile | 1185-53-1 | MFCD00012590 | 500mL
Tris-HCl Buffer 10mM, pH 8.0, Sterile | MW: 157.596 | 1185-53-1 | MFCD00012590 | 500mL
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 100mL
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Bioworld Tris-HCl Buffer 10mM, pH 8.5, 500ml
Common buffer for biological sciences. Technical Information: Biotechnology grade, pH 8.5 +-0.1. Warnings: Causes irritation to eyes, skin, and mucus membranes. Do not inhale or ingest. Wash thoroughly after handling. Appearance: Liquid, PH Value: 8.5, Molecular Weight : 157.6, Molecular Formula : C4H11NO3.HCl, pH : 8.5, Solubility : Soluble in water. Appearance Form : Liquid. Appearance Color : Clear. 500ml
Teknova 10X PBS Solution, pH 6.5 (at 1X). 1000 mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
STEMCELL Technologies RoboSep™ Buffer, Size: 250 mL
RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.4 - MB-002
Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution (tris(hydroxymethyl)aminomethane hydrochloride) is useful for stabilization of buffer formulations and electrophoresis of biological molecules. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.